A. Worker honey bees were restrained in plastic drinking straws using a

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Download scientific diagram | A. Worker honey bees were restrained in plastic drinking straws using a yoke made of insect pins placed on either side on the neck. Honey bees were supported from below using a rolled paper tissue. A small window was cut in the straw to allow full extension of the proboscis. B. Restrained honey bees were placed in front of individual light presentation screens. Each screen could be illuminated with a blue or green led and had a red LED mounted on top to indicate US presentation to the experimenter. C. A series of projection screens allowed simultaneous conditioning of up to ten honey bees. D. Both the rewarded and unrewarded trials used the same timing of CS/US presentation. Following a 3 sec countdown (not depicted), the CS presentation lasted 5 sec during the final 3 sec of which the US was presented. Proboscis extensions (responses) were recorded to the CS before and during the US presentation. from publication: Visual Associative Learning in Restrained Honey Bees with Intact Antennae | A restrained honey bee can be trained to extend its proboscis in response to the pairing of an odor with a sucrose reward, a form of olfactory associative learning referred to as the proboscis extension response (PER). Although the ability of flying honey bees to respond to | Honey, Bees and Associative Learning | ResearchGate, the professional network for scientists.