Step-by-step efficiency of cloning, small-scale protein expression and

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Download scientific diagram | Step-by-step efficiency of cloning, small-scale protein expression and purification of 271 conserved proteins of E. coli. A total of 220 purified proteins with high and moderate yields were subjected to large-scale expression. Afterwards, 176 proteins could be purified with a minimum of 500 g. Overall, 34 target proteins were subjected to Gateway recombination cloning to express fusion proteins with maltose binding protein, glutathione S-transferase, and N-utilizing substance A, respectively. Overall, 21 proteins fused to maltose binding protein were purified with a minimum of 500 g. ECHH, E. coli-human-homologues; LUCA, Last Common Universal AncestorDownload Power Point slide (282 KB) from publication: Identification of the predominant antigenic epitopes in intestinal flora in IBD | The normal intestinal flora is required for the development of intestinal inflammation in animal models of inflammatory bowel disease (IBD). In humans, several studies indicated a potential association of Escherichia coli (E. coli) with IBD. In addition, we have shown that | Inflammatory Bowel Disease, epitopes and Gastrointestinal Microbiome | ResearchGate, the professional network for scientists.

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Step-by-step efficiency of cloning, small-scale protein expression

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